Genetic characterization of strains of FMD virus in the Kyrgyz Republic
Project Status: 3 Approved without Funding
Duration in months: 36 months
Objective
Main objective of the project is carrying out the epidemiological monitoring, studying the separated isolate and its comparison with reference strains, identification of a foot-and-mouth disease virus genetic changes with the description of the morphofunctional characteristics of internal organs of animals during the disease.
To achieve this purpose it is necessary to solve the following problems:
- to carry out a monitoring of the foot-and-mouth disease of animals in recent years, mapping places of outbreaks using GIS;
- differentiation of the vaccinated livestock from infected, studying of 3ABC nonstructural proteins by the ELISA method;
- histology and immunohistochemistry investigation of internal organs of the fallen infected animals;
- identification of genome fragments of the virus of the foot-and-mouth disease from local isolates by means of the polymerase chain reaction (PCR);
- to carry out the phylogenetic analysis (sequence analysis) of the isolates;
- sequencing of structural proteins of the virus of the foot-and-mouth disease (VP1, VP2, VP3, VP4) and identification of amino acids responsible for a virulence.
- studying of a phenotype of nonstructural proteins of the virus of the foot-and-mouth disease and change of their features under the influence of a vaccine and field strain.
- to develop the program of monitoring and eradication of the foot-and-mouth disease of animals;
- training of experts from the Kyrgyz Republic – participants of the project in laboratories of EU, Japan, USA, Canada or other countries.
Participating Institutions
LEADING
Kyrgyz Research Institute of Veterinary named after A.Duysheev (KRIV)
