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Microwaves Effect on Calcium-Dependent Channels

#A-1750


The Study of Metabolic Pathway of Microwaves Effect on Calcium-Dependent Potassium Channels in Neurons and T Lymphocytes

Tech Area / Field

  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
  • BIO-SFS/Biosafety and BioSecurity/Biotechnology
  • MED-DIS/Disease Surveillance/Medicine
  • MED-RAD/Radiomedicine/Medicine

Status
3 Approved without Funding

Registration date
08.05.2009

Leading Institute
Life Sciences International Postgraduate Educational Center, Armenia, Yerevan

Collaborators

  • Yale University / Department of Pharmacology, USA, CT, New Haven\nResearch International, USA, NY, Williamsville

Project summary

Microwave (MW) therapy is the application of low-intensity millimeter-wavelength electromagnetic waves in the treatment of a large variety of diseases. In addition, significant knowledge accumulated about its immune-stimulating effects as well (Ayari & Balana-Cervero 2003; Ziskin 2006, Moazezi, Ayrapetyan 2008.). However, the cellular and molecular mechanisms leading to the immune-modulation remains completely unclear. Although the prevailing role of intracellular messenger-regulated Ca2+-dependent potassium channels in antigen-dependent activation and proliferation of T lymphocytes is also well documented (Panyi 2005), however the fundamental studies about the effect of MW on these ionic channels is not clear yet. Therefore, the overall aim of the present project is to elucidate the mechanism through which MW could modulate the activity of IK channels.

The scientific objective of the project is to find out the metabolic pathway through which the MW could modulate the functional activity of Ca2+-dependent IK channel activity in neuronal and T lymphocyte membrane. For this purpose the comparative study of low intensity (less than 5mW/cm2) MW and nM concentrations of H2O2 on a) biophysical properties of Ca2+-dependent K+ channels, by Voltage-clamp and Patch-clamp methods; b) Na+-K+ pump and Na+/Ca2+ exchanger, by measuring 86Rb & 45Ca uptakes, accordingly; c) intracellular level of cAMP and cGMP by immunoassay method, in neurons and T-lymphocytes would be performed.

Although the immune modulator properties of low intensity MW are well documented, its cellular and molecular mechanisms remain unclear. The literature data on pivotal role of Ca-dependent potassium channels in antigen-dependent activation and proliferation of T lymphocytes (Panyi 2005), and previous our data (Ayrapetyan 2006) on high sensitivity of IK channels to influences of MW and H2O2 (reliable messenger for biological effect of MW), allow us to suggest a working hypothesis, according which the intracellular messenger–dependent Na+/Ca2+ exchanger regulating Ca2+ dependent K+ channel activity in T lymphocyte membrane could serve as the metabolic pathways through which the immune activation effect of MW on organism is realized. Testing this hypothesis is one of the subjects of current research program. We hope that this study would be useful not only for understanding the therapeutic effect of MW stimulation, but also to estimate its potential in the generation of leukemia in children upon long-lasting MW exposure.


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