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Dispersed Aerosol Microbial Cells

#1296


Development of Technology to Obtain Fine–Dispersed Aerosol of Microbial Cells for Group Immunization of Human and Animals

Tech Area / Field

  • MED-DID/Diagnostics & Devices/Medicine

Status
3 Approved without Funding

Registration date
20.07.1998

Leading Institute
Research Center of Toxicology and Hygienic Regulation of Biopreparations, Russia, Moscow reg., Serpukhov

Project summary

The goal of the proposed project is:
1. Development of efficient technology for group aerosol immunization of human and animals.
2. Design of the universal installation to obtain high-concentrated fine-dispersed and stable aerosols of live vaccines.

To prevent a beginning and dissemination of infectious diseases in human and animals, the specific prophylactic measures are of paramount significance. Until the present, the most widespread ways for human and animals vaccination remain the methods of inpidual subcutaneous and jet injections. These methods require high sterility for used instruments and for the treatment procedure, as well as a large number of professionally skilled medical and veterinary personnel.

In the 50–80s the theoretical and laboratory studies for development of a new method for specific prophylaxis of infectious diseases as an aerosol immunization of human and animals have been carried out.

In spite of previous successful research, the method of aerosol immunization is still out of practice because of a number of reasons. The absence of efficient generators for obtaining high-concentrated fine-dispersed aerosols is one of the reasons for limited use of aerosol immunization.

We developed and successfully have explored the new original pneumatic generator of liquid aerosols (PGLA) as a unit of the vertical dynamic aerosol installation. The generator has a regulated consumption (from 1,0 to 30 ml/min) of dispersed suspension with output in aerosol (in dependence on microbial concentration in suspension) up to 108–109 m.cells/m3 and contents particles with size no more 10 m up to 99%, at the same time it has 80% particles up to 5 m. The generator has a single loading by dispersed suspension 0,5–10,0 litres (it depends on the purpose of the experiment) and can work persistently for several hours, creating and maintaining necessary concentration of m.cells/m3 in the limited volume (premises or aerosol chamber). Generator produces stables aerosol at 1–1,5 atm with compressed air pressure and output volume 50–60 l/min.

On the base of the PGLA-generator a laboratory pattern to carry out aerosol immunization of human and animals was developed at the Centre. Preliminary tests revealed its high effectiveness.

To reach the objectives of the project and resolve the problem of efficient protection of human and animals against especially dangerous infections by the method of aerosol immunization it is suppoused to solve the foollowing:


– to study technological regimes to obtain high-concentrated (108–109 m.cells/m3) fine-dispersed (no more 10 m) aerosol of microbial cells up to 90% (by weight) with use of the existing laboratory base (aerosol chamber installations, generators of aerosols and devices for their diagnostics);
– to design the universal installation to carry out the group aerosol immunization of human and animals;
– to improve the method to obtain the necessary microbial concentration of cells in aerosol indoor (or box) and to maintain it on the needed level during the whole immunization session;
– to develop an optimum immunization dose of vaccine using laboratory animals;
– to develop express-method for estimation of an immunization dose of microbial cells in aerosol for no more 30 minutes to determine a dose which an object will receive;
– to develop technology of group aerosol immunization of human and animals taking advantage of laboratory animals;
– to carry out comparative investigations of effectiveness of immunization of laboratory animals by injection and aerosol (with use of the developed installation) methods.

Expected Results

The follows will be developed as a result of carried out investigations:


– technology to obtain fine-dispersed aerosols of microbial cells with a part of particle up to 10 m no less than 90%, including particles up to 5 m no less than 50%;
– technique for express-evaluation of the proposed immune dose of microbial cells;
– methodical recommendations for group aerosol immunization of human and animals by live bacterial vaccines;
– experimental model of universal installation to carry out the group aerosol immunization of human and animals will be developed and designed.

The Potential Role of Foreign Collaborators

Foreign collaborators would take part in the project under the following directions:


1) patent-information support of the project;
2) methodology recommendations under obtaining fine-dispersed aerosol, improvement of the stability of microbial cells under their dispersion and flow in aerosol, as well as estimation of the impact on experimental animals;
3) publication of papers in periodic press abroad.


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