Photodynamic Therapy of Infections
Development of Effective Reactive Oxygen Generation Systems for Antimicrobial Photodynamic Therapy on the Basis of Cationic Photosensitizers and Coherent and Incoherent Light Sources
Tech Area / Field
- MED-DID/Diagnostics & Devices/Medicine
- PHY-OPL/Optics and Lasers/Physics
3 Approved without Funding
NPO Astrophysica, Russia, Moscow
- Moscow State University / Department of Biology, Russia, Moscow
- Centre Alexis Vautrin, France, Vandoeuvre\nHarvard Medical School / Wellman Center for Photomedicine, USA, MA, Boston
Project summaryThe basic purposes of the present project are the development of prototypes of incoherent and coherent light sources for excitation of photosensitizers, effective in antimicrobial photodynamic therapy, and carrying out the tests in vitro and in vivo to demonstrate killing of cultured pathogens and to cure infections.
The wide expansion of pathogenic microorganisms, resistant against antibiotic drugs, puts development of new effective therapies of infections on the agenda. One important future methodology is photodynamic antimicrobial chemotherapy (PACT) based on pathogen destruction by reactive oxygen species produced by the combination of non toxic chemical compounds - photosensitizer (PS) and corresponding irradiation with harmless visible light.
On the basis of available world experience in photosensitization of microorganisms, including authors of the present project, the following structural requirements for effective PS for PACT can be formulated: hydrophilic nature, cationic properties (especially for PS with wide antibacterial activity), long-wave absorption in far red or IR spectral region.
Development of light sources for photodynamic therapy of infections and their testing in systems in vitro and in vivo will be based on the available experience of a group of authors including development of sources of radiation, the laser and other light source equipment (SUE «SPA Astrophysica») and research in photophysics, photochemistry, photobiology and microbiology (Biological faculty the Moscow State University).
It is planned to solve the following tasks:
- Development of irradiators on the basis of sources of incoherent (lamps, light-emitting diodes) and coherent (lasers) radiation for laboratory tests of biological activity of synthesized PS and optimization of modes of light influences.
- Development of excitation systems for the most effective PS and creation of prototypes of devices for PDT
- Research of antimicrobial activity of PS and light sources in vitro on laboratory test cultures, including bioluminescent bacteria and yeast.
- The study of the effectiveness of active oxygen generating systems (PS and light source) in animal models infected with bioluminescent bacteria.
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