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Biocontaminated Areas Disinfection at Low Temperature


Working out and Estimation of New Ways and Methods of Area Disinfection Contaminated as a Result of Bioterrorism at Low Temperature

Tech Area / Field

  • BIO-SFS/Biosafety and BioSecurity/Biotechnology
  • CHE-OTH/Other/Chemistry

3 Approved without Funding

Registration date

Leading Institute
Research and Production Center for Environmental Monitoring "RACEM", Russia, Kirov reg., Kirov


  • Public Health Agency of Canada, Canada, ON, Ottawa\nUniversity of Ottawa, Canada, ON, Ottawa

Project summary

Many industrial and research facilities are constantly working with high-pathogenic and potentially dangerous infectious agents, representing the danger of their escape and outage. Such bio-agents have been already used and can be used to commit an act of terrorism in the future. To provide security to the population and environment it is extremely important to control the possibility to use the certified means and methods for effective disinfection of these bio-agents in field conditions under emerging of accidental and premeditated escape.

Though the available decontamination processing means and techniques are plentiful, only few comply with the requirements set for being used in real dangerous situations required decontamination. For instance, elimination of bio-terror act consequences in the USA when anthrax spores were used revealed plenty of serious problems related to efficacy of decontamination methods. In this regard, construction of the National Center for Analytical Methods of Bio-protection and Counter-measures was initiated in Frederick, Maryland, USA. The Center will carry out scientific research to reduce the hazardous consequences after the possible attack performed with the use of biological weapons. The Center will be opened in 2008.

In the case of bio-agents escape it is usually necessary to carry out premises decontamination at the temperature about 18 – 25°С. In the case of open-air decontamination considerable fluctuation in temperature of air and soil is expected, especially in regions with temperate climate. As a rule, lower temperature reduced the bactericidal activity of chemical reagents used for decontamination at the present time.

The purpose of our research is to create and to estimate such decontamination means and methods, which are able quickly to inactivate infectious bio-agents at lower temperature and under the field conditions. In this regard, the following researches are to be carried out:

  • to use model microorganisms to work out essential experimental methods in order to create more effective decontamination techniques for elimination of accidental and premeditated bio-agent escape;
  • to select more promising techniques and work out modes how to use the improved compositions for elimination of consequences after accidents and bio-terror attacks which were carried out using spore microorganisms;
  • to carry out experimental check of efficiency in application of the developed disinfectants under the model conditions (in vitro – using museum strains);
  • to carry out experimental check of efficiency in application of the developed decontamination aids, techniques and modes under the real conditions - enclosed spacious facilities, using model microorganisms.

Experimental check of efficiency in application of the developed decontamination means for dangerous pathogens will be held in Canada in the laboratory of the collaborator (in vitro – using museum strains).

Expected results and their application

Following will be done under the project:

The precise literature will be reviewed mainly on the problem of different experimental methods and data interrelation to test disinfectants at low temperature.

Collected information and knowledge will be used to improve disinfectant compositions and applied techniques to avoid influence of the negative temperature on the present compositions in the field conditions; such experiments are supposed to be carried out in the laboratory under different refrigerating temperature.

Experimental check will be performed to assess the decontamination efficacy of the selected techniques/methods related to different spore bacteria of 1 and 2 dangerous pattern (Bac. cereus. Bac. thuringiensis, Bac. anthracoides, Bac. anthracis). Received data will be acknowledged using 2-3 selected strains Bac. Anthracis (under the laboratory conditions of Ottawa).

Additional experiments will be held, experiments with disinfectants, using aerosolization, dispersion or atomized lavage under the negative temperature.

Experimental check on premeditated contaminated area will be performed using nonpathogenic substitute of dangerous pathogens. Environmental conditions will be investigated such as relative humidity, illumination, as well as air and soil temperature. Before the research soil samples will be collected and investigated on pH, moistness, organic substances and others.

As a final stage of the performed activities, guidelines on decontamination aids and techniques at low temperature will be originated.


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