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Biological Control of Pest Insects

#G-1464


Biological Protection of Horticultural Crops from Major Pest Insects

Tech Area / Field

  • AGR-PPR/Plant Protection/Agriculture
  • BIO-MIB/Microbiology/Biotechnology

Status
3 Approved without Funding

Registration date
06.10.2006

Leading Institute
Georgian Kanchaveli L. Research Institute of Plant Protection, Georgia, Tbilisi

Collaborators

  • Ohio State University / Ohio Agricultural Research and Development Center, USA, Wooster, Ohio\nChristian-Albrechts-Universität Kiel / Institute for Phytopathology, Germany, Kiel\nEuropean Biological Control Laboratory, France, Montferrier sur Lez\nAgriculture and Agri-Food Canada / Lethbridge Research Centre, Canada, AB, Lethbridge\nAgriculture and Agri-Food Canada, Canada, QC, Saint-Jean-sur-Richelieu

Project summary

The main goal of suggested project is to search and identify local pathogens in the populations of target pest insects and to accomplish plant protection in horticulture by using the selective bioformulations (on the base of entomopathogenic bacteria, fungi, nematodes) and recommend opportunities for mass production in Georgia, where the damage by pest insects in several agricultural systems has achieved the large scale.

Georgia does not produce the biological means and the import from foreign countries connects with high costs. As a results of investigations the bank of pathogenic organisms of pest insects will be create. After project finishing it'll be possible to submit the selective strains of organisms to mass production. This will certainly contribute to the progress in various fields (agriculture) of national economy.

The development of local produce industry of plant protection biological means gives the possibility to reduce the costs connected with the import of the analogical bioinsecticeds from foreign countries, and no chemical pesticides will be imported, which negatively influenced on environment. These provide the preservation of biopersity and ecological tolerance, the obtained of pure products and generally the rise of national economy. Project serves to the restoration and preservation of the positive ecological balance.

Through implementation of the project many significant results are expected: this project develops biological control approaches to manage the fall webworm, the Colorado potato beetle, the gypsy moth, the codling moth and etc. having significant impact on horticulture in Georgia. In addition, because few pest controls are currently being used, this is an ideal system to establish an IPM/biological control approach to compete with heavy pesticide usage. The small plot farming systems are also amenable to research in this area. The results will be very important for elaboration of biological plant protection from not only the above mentioned species but other Lepidoptera, Coleoptera, Homoptera pest insects introduced in the territory of Georgia. The results will have great practical importance for biological control of pest insects generally in Caucasus region, for IPM approaches and for distribution of electronic and published information on them. However, currently there are no locally produced biological formulations. Although it can be expensive to initiate production, biological plant protection can be very important for human and environmental safety.

The suggested proposal has some novelty for Georgia. The availability of biological formulations (and perhaps the other strategies) which provides reasonable control with low mammalian toxicity could result in widespread use in Georgia, just as in the US. This alone would likely spur more interest in the sciences related to pest control.

The results of the project could serve as the basis for new or improved technology, have potential commercial value in the marketplace, where the ecological pure products obtained under the this project implementation will take on added value, and assist in the solution of societal problems, especially in the development of the Georgian economy. Farmers and growers of agricultural products will benefit directly from the results of this project.

This project will substitute new for application of existing knowledge and processes. We expect that the proposed project will provide the basic data and experience for the extensive use of biological control agents in Georgia and provide an effective alternative to hazardous chemical pesticides. Indirectly, it will help to open new markets for agriculture products in the Caucasus countries.

The implementation of the project will make possible for "weapon" scientists and experts to redirect their abilities and knowledge to peaceful activities, fostering the process of Georgian scientists integration into scientific community; the development of progressive technologies in mass production and using of pathogenic organisms for peaceful purpose, such as save of environment from pollution. The implementation of the Project will cover the objectives sought by ISTC.

The implementation of this project involves the following work to be performed in stages:

  1. Conduct and analyze laboratory experiments on imported biological formulations on the larvae of pest insects using methods generally accepted in insect pathology;
  2. Examination of biological formulations in: Orchards, Citrus plants, Vegetable gardens, Closed environment;
  3. Organization of the proper techniques, problems and solutions for accomplishing biological plant protection from harmful insects.

Foreign collaborators (USA, Europe) agree to cooperate in scientific research work provided by the project, to exchange information in the course of its realization, to give consultations, to evaluate the obtained results, to carry out joint discussions of the obtained data, to invite and visit project participants. Consulting and methodological aid in the choice of modern methods and technologies of investigations. Assistance for the Project participants in attending International symposia and conferences.

The experiments in laboratory and natural conditions will carry out by using of local pathogenic strains and introduced formulations. The phenological calendars on each target insects will be compiling. The local and introduced entomapathogenic nematodes, Steinernema feltiae will be rearing in vivo on the Galleria mellonella last instars larvae. The recent technique of application technology of biological formulations will take the important place in the integrated pest management (IPM) in several ecosystems (Orchards, Citrus plants, Vegetable gardens, closed environment).

The searching of local pathogenic organisms at the populations of pest insects in the several agroecosystems will be conducted mainly to entomopathogenic nematodes such as described in recent scientific literature (Neher, Wu, Barberchek, Anas, 2005; Mracek, Becvar, Kindlmann, Jersakova, 2005). The commercial bacterial formulations containing Bacillus thuringiensis (novodor, xentar, dipel) will be used in experiments. The strains of entomopathogenic fungi Beauveria bassiana (stored in EBCL, France) will be used as separately as joint to EPNs for experiments in laboratory and field conditions. The local fungi strains of the Japanese scale, Lopholeucaspis japonica (Cockerell) – Aschersonia sp. will be used in experiments (Tabatadze, 2000). The investigations of entomopathogenic organisms will carry out by using the methods generally accepted in insect pathology (Weiser, 1977; Manual of Techniques in Insect Pathology, 1977). To establish the baseline efficacy (LD50, LT50, etc.) in bioassays for pest insects generally accepted statistical methods have been used as described in “Methods for analysis of data from bioassays with insect pathogens” (Throne, 2005). The recommendation of using of biological formulations for plant from harmful insect will be compile and submit to farmer's holdings.


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The International Science and Technology Center (ISTC) is an intergovernmental organization connecting scientists from Kazakhstan, Armenia, Tajikistan, Kyrgyzstan, and Georgia with their peers and research organizations in the EU, Japan, Republic of Korea, Norway and the United States.

 

ISTC facilitates international science projects and assists the global scientific and business community to source and engage with CIS and Georgian institutes that develop or possess an excellence of scientific know-how.

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