Selective DNA Biosensors with High Output Signal Amplified by Duplex-Specific DNA-Ligand Binding and their Application to Prevent Terrorist Threats
Tech Area / Field
- BIO-SFS/Biosafety and BioSecurity/Biotechnology
3 Approved without Funding
Yerevan State University, Armenia, Yerevan
- Aachen University of Applied Sciences / Institute of Nano- and Biotechnologies, Germany, Jülich\nUniversity of Toronto / Faculty of Pharmacy, Canada, ON, Toronto\nUniversite de Paris-Sud / Laboratoire SIMA associé à l'Université de Paris-Sud, France, Gif-sur-Yvette Cedex\nAuburn University / Materials Research and Education Center, USA, AL, Auburn\nKorea Atomic Energy Research Institute / Advanced Radiation Technology Institute, Korea, Jeongeup\nUniversity of Uppsala / Department of Engineering Sciences, Solid State Physics, Sweden, Uppsala\nKorea Institute of Energy Research (KIER), Korea, Yusong-gu
Project summaryElectrochemical DNA-sensors can be applied for monitoring of environmental pollutants and in the food industry, defense and security as well and, especially, for bioterrorist threat detection because of them high reliability, usability and low cost.
The main idea of the present Project consist in using the DNA duplex specific binding of some ligands, possessing specific electric and optic features to enhance the output signal of electrochemical DNA-sensors. At the same time duplex DNA-ligand binding, will result in stabilization of the double-stranded structure of DNA and thereof to improvement of an output of a signal of DNA-sensor. Application of duplex-specific intercalators such as e.g. porphyrins will permit to enhance the electric signal generated by DNA hybridization event. Besides, usage of new intercalators with higher affinity to double strand DNA should make easier registration of the hybridization events on substrate at the expense of generation of new types of signals. On the basis of differences between binding constants for DNA with different ligands and the degree of DNA denaturation depending on external factors, such as pH, ionic strength etc., conditions providing the multiple using of DNA–sensors will be elaborated.
The electric signal generation, caused by target compounds recognition is linked with semiconductor substrate bearing the DNA probes and at the same time applying as an electrode. We propose to use semiconductor materials, due to their abilities to operate long term under physiological conditions, high biocompatibility, inertness to biological tissues and aggressive environment, and the possibility of their application for constructing of all types of electronic devices. Among such materials we prefer to use porous silicon, degenerated non-stoichiometric metal oxides (TiO2, SnO2 etc.), silicon carbide, diamond like carbon films. Possibilities to make thin film substrates for sensors on single- or multicrystalline silicon allow manufacturing integral biosensitive circuits, which can be included immediately in large integral circuits and be an inseparable part of chips.
DNA biosensors' selectivity, sensitivity, and ability to display processes taking place in the cell will be proved in comparative experiments on cellular models in vitro.
The prototype of DNA-sensor on the basis of semiconductor materials will be developed.
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