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Detoxication of Soils Contaminated by Obsolete Pesticides


Demonstration of Integrated Technology for Detoxication of Soils Contaminated by Obsolete Pesticides

Tech Area / Field

  • BIO-IND/Industrial Biotechnology/Biotechnology
  • BIO-MIB/Microbiology/Biotechnology
  • CHE-SAS/Safety and Security/Chemistry
  • ENV-EHS/Environmental Health and Safety/Environment
  • ENV-RED/Remediation and Decontamination/Environment

3 Approved without Funding

Registration date

Leading Institute
Department of Chemicalization, Protection and Quarantine of Plant, Kyrgyzstan, Bishkek


  • International HCH & Pesticides Association, Denmark, Holte\nObihiro University of Agriculture & Veterinary Medicine, Japan, Obihiro

Project summary

Today detoxication of soils at the Kyrgyz Republic is an urgent problem because of contaminated with the forbidden pesticides soils (soils where persistent organic contaminants pesticides (POC-pesticides) were used), agricultural aviation airdromes and sites of their location, former storages of pesticides) are used for the different crops growing.

Decontamination of soils from pesticides is the mostly difficult method of waste disposal due to the complicacy of the properties and variety of soils and concentrations of the pesticides into soils. In order to separate pesticides from soil on the site of contamination the following methods are used: high-frequency heating, electro-kinetic treatment, and soil washing out and chemical treatment with polyethylene glycol and KOH, and zero-iron treatment. However, direct soil treatment by high-frequency heating for soil regeneration at Kyrgyzstan is not suitable because of economic, geographic and technological difficulties of the specific sites contaminated with pesticides. This conclusion can be made on the base of pesticide inventory performed by the State Department of Chemicalization, Protection and Quarantine of Plants of Kyrgyz Republic in framework of completed ISTC project KR-886. It should be noted additionally that all abovementioned methods of pesticide separation from the soil could not be applied in small farms because of high cost of the equipment required for these aims, and not environmentally safe at the chemical treatment.

The proposed in project the integrated detoxication technology will be include the soil pretreatment using formulations based on microorganisms of pesticide destructors and nutrients amendments to stimulate the microbial metabolism and follow-up phytoremedition, i.e. exploit native plant species and their associated rhizosphere microbial communities to absorb and accumulate pesticide residues. Currently, phytoremediation technology are not used in Kyrgyzstan. The technology has the potential to be profitable due to the inherent climatic characteristics of the Republic. The Kyrgyz climate allows for a long vegetative period of plants and goes from March till November, thus favoring optimal conditions for soil microflora development. Phytoremediation technology will be used in an environmentally friendly manner in situ i.e., on places conferring ecological safety for plants and living organisms, especially where costs of project implementation and its maintenance are reasonably low.

A key point during the development of phytoremediation technology includes the identification of a suitable plant species that is adapted and capable to grow in heavily polluted sites. Additionally, a few steps can be introduced during the process to accelerate degradation of pesticides in the soil. These include: (i) Cultivation of adapted plant species in the area, (ii) Establishment of optimal environmental conditions for intensive crop growth; and, (iii) Increasing the metabolic activity of rhizosphere micro-biocenosis by using biological agent (microbial inoculants) formulations concurrently with plants.

The objective of the proposed project is to demonstrate the integrated biophytoremediation technology of soils contaminated with forbidden and non-serviceable pesticides in agriculture. In order to achieve our goals, the following tasks need to be solved:

  1. To inspect the soil model sites in the places of former storages of pesticides, dumps and agricultural aviadromes to assess the morphological, physicochemical and biological properties of soil. Creation of data base for soil characteristics obtained.
  2. To develop technology for obtaining microbial agents based on high-efficient microorganisms strains-destructors degrading pesticides in lab conditions. To study the efficacy of microbial degradation of pesticides in contaminated soil using microorganisms-destructors in lab conditions.
  3. To develop biotechnology for obtaining microbial agents based on beneficial rhizosphere microorganisms tolerant to environmental stresses and exhibiting growth-stimulating activity associated with plants – pesticide accumulators. To conduct remediation studies in lab (with hydroponic culture, using seedling technique, and pot experiments) and field experiments for development of schedule of tests.
  4. To perform the toxicological and phytotoxic assessment of bioagents based on promosing microorganisms strains with pesticide-destructive and PGPR activity.
  5. To develop the lab and pilot-plant technology for the production of microbial agents - destructors of pesticides and plant growth stimilators – pesticide – accumulators.


The International Science and Technology Center (ISTC) is an intergovernmental organization connecting scientists from Kazakhstan, Armenia, Tajikistan, Kyrgyzstan, and Georgia with their peers and research organizations in the EU, Japan, Republic of Korea, Norway and the United States.


ISTC facilitates international science projects and assists the global scientific and business community to source and engage with CIS and Georgian institutes that develop or possess an excellence of scientific know-how.

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