Diagnostics of Herpetic Tissue Changes of Human
Automated Diagnostics of Herpetic Tissue Changes of Human Organs by Histological and Cytological Image Processing
Tech Area / Field
- INF-SOF/Software/Information and Communications
- MED-DID/Diagnostics & Devices/Medicine
3 Approved without Funding
National Academy of Sciences of the Republic of Belarus / Institute of Informatics Problems, Belarus, Minsk
- Belarusian State Medical University, Belarus, Minsk
- University of Essen / Institute of Pathology and Neuropathologie, Germany, Essen\nSunnybrook & Women's Colledge Health Sciences Centre, Canada, ON, Toronto\nUniversity of Nottingham / School of Computer Science & Information Technology, UK, Nottingham
Project summaryThe purpose of proposed project is the elaboration of methodology, algorithms and software for computer system for diagnostics of lesions of human organs due to herpes simplex virus (HSV).
The state of things the art in the field of the diagnostics of herpetic lesions. Problem actuality. The herpes is the most spreaded among all virus infections. More than 90% of human population is contaminated by HSV and 20% of them have clinical manifestations of herpetic infection. According to the information of WHO the mortality caused by herpetic infection occupies the second place after influenza [Booss J., Esiri M.M. Virtual encephalitis. – Oxford Blackwell scientific publication]. The evolution of ideas about relation of HSV and human organism has taken place on the base of study of peculiarities of herpetic infection over the 80 years. At the XXth century it was proved that the reproduction of HSV hurts not only skin and mucous membranes but different organs and systems of human organism [Mandell, Douglass and Bennett’s principles and practice of infections diseases. By G.L.Mandell, J.E.Bennett, R.Dolin.- 4th ed, Churchill Livingstone inc., -vol.2,1995]. The affections of nervous system, the heart, the liver, and the lungs are more dangerous. The experimental and clinical study of herpetic infection have allowed to establish the fact that different forms of herpetic infections are often accompanied by formation of immune deficit in human organism [Scoldenberg B. Herpes Simplex encephalitis // Scan. J. Infect. Dos.- 1996.]. The modern methods of diagnostics and monitoring of herpetic lesions are of complex character and demand participation of different specialists. To increase accuracy and accelerate deasease diagnostics, the morphological analysis of organ’s tissue is the most important. Therefore the task of automation of morphological diagnostics of herpetic lesions is very important in medicine. The proposed computer system for morphological diagnostics of herpetic lesions improves accuracy of diagnostics and compensates deficit of morphologist-specialists. This system has no analogs.
The impact of the proposed project on the progress in the field of clinical diagnostics. The expected results of the project implementation are important in respect to extending an international scientific cooperation in the field of diagnostics of the herpetic lesions, and the reasons for that are the following:
- Improving quality of diagnostics of herpetic lesions. The introduction of the unified estimation approaches to the degree of the lesions due to HSV of patients for different population groups will allow to detect specific elements of lesion evolution.
- Decreasing risk of incorrect diagnostics by automation of morphological diagnostics of herpetic lesions.
- Compensation of deficit of specialists, who perform the diagnostics of herpetic lesions. Computer system for morphological diagnostics of herpetic lesions will facilitate the diagnostics. Physicians will be able to consult with experienced specialists with the help of a teleconsultation module.
- Creation a unique database of lesions of human organs affected by herpes simplex virus.
- Improving training of specialists by electronic atlas of images of histological preparations with herpetic lesions.
- Methods of automated morphological diagnostics of herpetic lesions of human organs will be applied in other systems of computer diagnostics.
Participants of the project. The project will be performed by teams of UIIP and BSMU with 18 participants. The UIIP team consists of 11 participants, including 5 weapon experts in the field of the new information technologies of computer processing, analysis, recognition and identification of cartographical images for the purpose of decision making in systems of missile pointing and control. The BSMU team consists of 8 participants, including 3 weapons experts in the field of viral diseases and biological weapon. The UIIP and BSMU participants have successfully performed research-and development projects during the last decade. Now the project participants make an attempt actively to turn from weapon oriented image processing to medical histological and cytological images of herpetic preparations for the purpose of creating an automated diagnostic system.
Expected Results. Category of Technical Development – technology development and demonstration.
The following results will be obtained within the project:
- a prototype of computer system for automated morthological diagnostics of herpetic lesions with an archive of histological preparations;
- novel algorithms and software for processing of images of cells and cell structures, which are oriented onto diagnostics of herpetic lesion;
- technique for herpetic lesion analysis of histological tissues of human organs and information technology of ascertainment a degree of herpes virus lesion.;
- digital atlas of histological images of herpetic lesions of human organs (heart, liver, lungs and nervous tissue) with support of telediagnostics system and WEB-publication;
The computer system for automated morphological diagnostics of lesion of human organs by herpes virus will be developed during the project period.
Scientific novelty of the project consists in developing of new methods of morphological diagnostics of herpetic lesions of human organs with the aid of histological and cytological image processing and a computer system of automated diagnostics.
Practical value of project results consists in application of computer system for improvement of diagnostics quality and accuracy as well as in compensation of deficit of specialists in the field of interest.
The system to be developed will be available for commercial distribution in medical institutions, while the methods and technologies to be developed can be applied in products for medical diagnostics.
Application of Results:
- Computer system can be used for accomplishment a clinical diagnostics of diseases in histological and cytological laboratories.
- Carrying out teleconsultations for herpetic lesions with the aid of digital atlas increases an effectivity of cooperation between specialists.
- Dgital atlas can be used efficiently for the teaching in medical institutions.
- A commercial distribution of the system and its parts in medical institutions is possible.
Meeting ISTC Goals and Objectives. Realization of the project will allow for weapon oriented participants to:
- redirect their talents, knowledge and skills related to weapons of mass destruction to peaceful activities in the field of problem development of automated clinical diagnostics of herpetic diseases;
- be integrated into the international scientific community by means of contacts and intercommunication with foreign scientists and specialists due to a joint activity in the field of diagnostics automation of viral diseases;
- contribute to the solution of national or international technical problems in the field of development of new automated technologies of viral herpetic disease diagnostics;
- stimulate a transition to market-based economics by creating a commercial product for automated diagnostics of viral herpetic diseases.
Information About an Effort:
The project is planned to be executed in 36 months in effort of 7326 person*days. The project team from UIIP will develop a prototype of diagnostic complex, algorithms for histological and cytological object extraction from images and calculating their characteristics, digital atlas of herpetic lesion images with support of integration with telediagnostics system and WEB-publication. The prototype will consist from eight program packages in total. The project team from BSMU will be engaged in collecting and processing of infectious materials. More than 1000 histological preparations of human organs (heart, liver, lungs and nervous tissue) from more than 400 patients. Furthermore, methodological approaches to the diagnostics of herpetic lesions and working out with protocols and structures for storing of required information will be developed by the specialists from BSMU on the basis of morphological investigation. Format of uniform card, methods and ideology of classification of histological objects in images, expert shell, digital atlas with all modules will be elaborated jointly.
Role of Foreign Collaborators.
Cooperation with three foreign collaborators will provide the following:
- information exchange in the course of the project implementation;
- provide comments to the technical reports (quarterly, annual final, etc.), submitted by the project participants to the ISTC;
- cross-checks of results obtained in the course of the project implementation;
- conduction of joint seminars and workshops in the course of the project implementation.
Collaborators from Sunnybrook and Women's College Health Sciences Centre (Toronto, Canada) and Institute for Pathology of University Hospital of Essen (Germany) ordered to realize:
- testing and evaluation of the diagnostics technology, developed in the course of the project.
The collaborator from the School of Computer Science and Information Technology University of Nottingham (UK) agreed:
- to lead testing and evaluation of the histological image processing technology, developed in the course of the project.
Technical approach and methodology. The researchers of UIIP have a wide experience in developing of weapon technologies and software for image processing, analysis and recognition. Besides they have an essential back-log of knowledge of methods and algorithms elaboration in the field of medical image processing. This experience is to be extensively used for solving project tasks. The participants from BSMU have wielded morphological methods of virus diseases detection and recognition over the time of last decades. The standard methods of histological material preparation will be used but with different colors for initial data obtaining. Novel methods of morphological diagnostics of herpetic lesions, as well as algorithms for cells extraction in images of different optical magnification and algorithms of cell structure segmentation and identification are to be developed and patented in the framework of the project. The testing of methods to br developed and system will be provided by colleagues of BSMU group on the base of city pathologoanatomy department of Minsk. The quality of the diagnostics will be controlled with immunohistochemical methods. The Collaborators will carry out a qualitative assessment of method and technologies to be developed.
The International Science and Technology Center (ISTC) is an intergovernmental organization connecting scientists from Kazakhstan, Armenia, Tajikistan, Kyrgyzstan, and Georgia with their peers and research organizations in the EU, Japan, Republic of Korea, Norway and the United States.
ISTC facilitates international science projects and assists the global scientific and business community to source and engage with CIS and Georgian institutes that develop or possess an excellence of scientific know-how.