Biochips for Tuberculosis Diagnostics
Use of biochips in the diagnostic and estimation of effectiveness of early treatment of poly resistant tuberculosis
Tech Area / Field
- MED-DID/Diagnostics & Devices/Medicine
8 Project completed
Senior Project Manager
Weaver L M
Kyrgyz Scientific Research Institute of Tuberculosis, Kyrgyzstan, Bishkek
- National Center of Cardiology and Internal Medicine, Kyrgyzstan, Bishkek
- Imperial College London/Department of Infectious Diseases and Microbiology, UK, London
Project summaryThe purpose of the project - study of abundance of antibiotic-resistant forms of tuberculosis by method of biological microchips (microarrays) for early diagnostics and estimation of efficiency of treatment of patients with this pathology by the ozone therapy in Kyrgyz Republic.
Now Kyrgyz Republic belongs to number of the states with poor epidemiological situation on abundance of polyresistant tuberculosis. Since 1991 noticeable increase of all epidemiological activities on tuberculosis (case rate, morbidity and mortality) began to be observed. Among investigated patients the drug resistant forms of a tuberculosis amount 79.0% in 2000 for the primary detected patients.
Much more often are registered the severe, spread, fast progressing forms of a tuberculosis with massive excretion of microbe frequently resulting in the lethal outcome. On a background of increase of the rate of the severe, spread, fast progressing forms of tuberculosis with massive excretion of microbe frequently resulting in the lethal outcome the number of a tuberculous infection by the polyresistant strains of Mycobacterium tuberculosis is annually increased.
Till now the diagnostics of tuberculosis remains a severe problem in a phthisiatry. The tuberculin tests are not very specific, rentgenologic methods and microscopic analysis are insensitive to detect already neglected forms and the bacteriological cultivation requires a lot of time. Especially at the abacillary form of a pulmonary tuberculosis and at extrapulmonary specific lesions with the “defaced” pattern of the process, the above described survey methods are poorly informative for early diagnostics. Practically always they will be utilized only for confirmation of the diagnosis “an active tuberculosis ”, not for its detection. The early diagnostic of resistant forms of tuberculosis is frequently based only on a clinical pattern and collection of indirect signs.
Now for identification of microorganisms and Mycobacterium tuberculosis (MBT) is often used a polymerase chain reaction (PCR) method. It is highly specific and extremely responsive test with the help of which it is possible to identify the presence of a unique DNA molecule of a pathogen. The PCR method is permanently perfected and now already has found its place in diagnostic of tuberculosis.
The recently designed method of biochips is having higher performance and appropriate the modern requirements of diagnostic. The use of oligonucleotide microchips underlies in a basis of this method. The given technology allows to identify mutations in the different Mycobacterium tuberculosis strains resistant to different drugs. It is known, that the mutations, responsible for development of resistance are in different genes or different sites of one gene (for example, mutations in genes pnsA, rpoB, rpsL, embB, katG, ndh, cause resistance to Pyrazinamidum, Rifampicinum, Streptomycinum, Ethambutolum, Isoniazidum, respectively). In this time these mutations are well studied, and the obtained results will be widely used for diagnostic of the disease. The important advantage of this method is a fast generation of a result compared to the bacteriological tests.
We offer the application of different techniques of ozone therapy in complex treatment of the tuberculosis patients.
It is known, that the effect of ozone on human organism at certain concentrations is accompanied by change of a metabolism, rise of partial oxygen pressure in blood, amplification of oxidation-reduction processes bound with generation and utilization of power resources (Crebs cycle), and also acceleration of activity of immunocompetent cells and inclusion of endogenic disintoxication mechanisms.
The published data on the effect of ozone on microorganisms are poor. However, it is known, that usage of ozone-oxygen mixture is effective concerning pyogenic flora, tuberculosis agent, diphtheria, gas gangrene (Apsatarov E., 1994; Vasil'ev I.T., Markov I.N., 1992).
The high performance of ozone-oxygen mixture concerning Mycobacteria Tuberculosis is confirmed also by experimental researches (Priymak A.A., et al., 1991; Belyanin I.I., et al., 1997).
Belyanin I.I. in 1997 has applied successfully an ozonized solution by the way of intravenous drop infusions at treatment of progressing pulmonary tuberculosis.
With the purpose of rise of efficiency of treatment of the different forms of pulmonary tuberculosis we offer the usage of the patented method (patent No. 227 from 30.11.97 Kyrgyz Republic) on a background of reference antibacterial treatment. The given technique of treatment allows achieving maximal recovery, even if there is a drug resistance of Mycobacterium tuberculosis.
The method provides immediate effect of ozone on microorganisms localized in the broncho-pulmonary system, and the application of other ways of ozone therapy allows curing tuberculosis of extrapulmonary localization.
Our jointly designed project promotes early detection of tuberculosis, rise of efficiency of its treatment and complete recovery of patients.
It is offered to receive the following results:
– to reveal the abundance of the rifampicinum-resistant forms of a tuberculosis with the use of a f microchips assay;
– to study the new effective methods of non-medicamental treatment of patients with pulmonary and extrapulmonary forms of tuberculosis with the help of ozone therapy (patent No. 227 KR);
– improvement of epidemiological activities on tuberculosis in Kyrgyz Republic.
On the given theme we conducted certain work. More than 50 patients with pulmonary and extrapulmonary forms of tuberculosis were treated by patented and some other ways of ozone therapy. As a result of treatment of patients conducted by us there was an acceleration of periods of germ clearing for 0.5 months and at rentgenologic control through 1-1.5 months it was observed the positive dynamics for 100% of patients towards a resorption of infiltration changes and closing of cavities of decay in lung. At extrapulmonary tuberculosis the application of appropriate ways of ozone therapy was accompanied by positive dynamics too.
The results of treatment by ozone therapy techniques are presented in publications (Kojomkulov J.K. et al., 1996; Alisherov A.Sh., Kojomkulov J.K. et al., 1998; Kojomkulov J.K. et al., 2000).
The study will be performed by scientists - in past working in military research.
The International Science and Technology Center (ISTC) is an intergovernmental organization connecting scientists from Kazakhstan, Armenia, Tajikistan, Kyrgyzstan, and Georgia with their peers and research organizations in the EU, Japan, Republic of Korea, Norway and the United States.
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