Cereal Crops in Tajikistan
Genome Analysis of Cereal Crops and Their Relatives in Tajikistan
Tech Area / Field
- BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
8 Project completed
Senior Project Manager
Weaver L M
Institute of Plant Physiology and Genetics, Tajikistan, Dushanbe
- Thomas Jefferson University, USA, PA, Doylestown
Project summaryTajikistan is one of the primary centers of origin of cultural plants (N.I.Vavilov). There are a lot of endemics here. Generally, endemics are the main source for creation of National Gene pool of crop plants using modern biotechnology approaches. Alongside with endemics the local, traditionally cultivated grades of plants created in research institutes and by inpidual breeders, make a gene pool, which is the property of the state. A collection of such grades may represent great genetic potential for selection. A problem of maintenance of cultivated grades is to collect them, analyze and use in biotechnological work on improvement of existing and synthesis of new grades of plants. Wild relatives of cultivated plants can be used for creation of new grades by genetic engineering and biotechnology methods to preserve biopersity of cultural plants.
The goal of the proposed project is studying of the genetic structure of various populations of cultural plants and their wild relatives in various ecological zones of Tajikistan and organization of the collection center on preservation and duplication of rare and valuable native genotypes of cultural plants in the Republic. It’s planned to preserve in the culture especially valuable genetic resources of Tajikistan and to elaborate technologies for long-term storage and mass propagation in order to restore violated natural populations, utilization for selection purposes, for plantation and domestication.
Institute of Plant Physiology and Genetics has all necessary resources for implementation of such project including specialized laboratories to do this work (molecular biology and gene engineering lab, genetics of photosynthesis lab, selection and others). The project participants are highly qualified specialists, who are working on the genetics of crop productivity, studying molecular characteristics of genetically modified crop cultures resistant to wreckers, viruses, drought, water deficiency, and can use the biochemical, molecular-genetics methods as protein analysis, PCR, RLFP, etc. Partial collections of valuable crop plants were previously established at IPPG that can be further characterized and utilized for creation of proposed genetic collection of crops.
Within the framework of the project we intend to do on 4 directions:
1. Creation a gene pool, gathering and protection of valuable wild-growing relatives, age-old samples and cultivated in Tajikistan cereal cultures;
2. Catalogue of crops genome;
3. Creation of bank of germ plasm;
4. Molecular characterization of crops genome and its integration in available world genetic resources.
Creation of collection center of crops in Tajikistan is necessary for storage of seeds, deep-frozen tissues, embryonic and somatic cells of plants and animals, suitable for the subsequent reproduction of representatives of kinds, grades, occurred in this region.
The control and verification of samples in proposed collection crops center will be carried out using modern molecular biology techniques, including polymerize chain reaction (PCR) and restriction length fragment polymorphism (RLFP) so it could be integrated in the world databases of genetic resources.
The International Science and Technology Center (ISTC) is an intergovernmental organization connecting scientists from Kazakhstan, Armenia, Tajikistan, Kyrgyzstan, and Georgia with their peers and research organizations in the EU, Japan, Republic of Korea, Norway and the United States.
ISTC facilitates international science projects and assists the global scientific and business community to source and engage with CIS and Georgian institutes that develop or possess an excellence of scientific know-how.