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Bionanotechnology for Bacteria Detection

#3245


Bionanotechnological Analysis of Affine Surfaces

Tech Area / Field

  • INS-DET/Detection Devices/Instrumentation
  • BIO-MIB/Microbiology/Biotechnology
  • BIO-OTH/Other/Biotechnology
  • ENV-WPC/Water Pollution and Control/Environment

Status
8 Project completed

Registration date
07.04.2005

Completion date
19.07.2010

Senior Project Manager
Genisaretskaya S V

Leading Institute
State Research Center for Applied Microbiology and Biotechnology, Russia, Moscow reg., Obolensk

Supporting institutes

  • ITEF (ITEP), Russia, Moscow

Collaborators

  • University of Groningen / Department of BioMedical Engineering, The Netherlands, Groningen\nTechnische Universitat Kaiserslautern, Germany, Kaiserlautern\nJohannes Kepler Universitat Linz / Institute for Biophysics, Austria, Linz

Project summary

This project is devoted to the development of bionanotechnological approaches for supersensitive biosensor systems construction on the base of affine surfaces. This approach will allow to analyze and to control of recognition system of biosensor for supersensitive bacteria detection and will be very important in a wide range of disciplines, including medical analysis, food and environment. We propose to develop unique nanosystem for bacteria and spores detection on the base of reconstructed AFM imaging, mathematical treatment of which will allow to visualize and characterize quantitatively single interaction antigen/antibody where nano-fragment of biological object, less than cell, will be used as antigen. Even though single cells in sample will give many fragments that will be detected safely by use proposed method. So new bionanotechnological approach will allow to develop supersensitive system for bacteria detection with sensitivity more than one bacteria.

SRC AM has had a core competition in microbiology and biosensors creation. Works in the field of methods of bacterial detection are intensively conducted. SSC RF ITEP is leading organization in analysis of samples of small size (atomic force/scanning tunnel microscopy and other). Now we collaborate intensively in field of development of nano-analytical systems for bacteria detection. The results of this investigations were published in international per review journals (Biosensors and Bioelectronics) and were presented at different conferences (including NATO ARW and NATO ASI).

The results of investigations will allow us to control the nano-organization of affine surfaces and to use the finding for the creation of supersensitive system for bacteria detection. This results will give us the real possibility to develop and use in practice bionanotechnologies connected with use of biosensors for fast and sensitive analysis of different bacteria.

Project provide for performance following stage:

  1. Analysis of nano-organization of different types affine surfaces (protein, lipid and polysacharide).
  2. Analysis of nano-organization of affine surfaces at different types of substrates (glass, mica, graphite and gold).
  3. Investigation of different types of immobilization of recognizing elements at the substrates for increasing of sensitivity of sensor system.
  4. Mathematical reconstruction of three dimensional imaging of specific interaction of recognizing element and analyt.
  5. Development of supersensitive method of biological fragment detection based on the evaluation and parameterization of reconstructed imaging by use of atomic force microscope.

This Project is in full compliance with the ISTC goals and objectives. As a result of the project scientific and technical personnel, which was occupied by weapon production and testing, will be orientated on a peaceful activity - the development of bionanotechnological approach for supersensitive bacteria detection The proposed project belongs to the area of applied and basic researches. However, its outcome will make a certain contribution to the development of bionanotechnological approaches in microbiology and creation of new analytical systems.

The role of foreign collaborators in the project - joint forming, discussion and correction of intermediate and final results, some joint investigations and developments, marketing assistance, possible subsequent joint use of developed technologies for bacterial analysis. Special attention will be noticed to the development and realization of bionanotechnological approaches for bacteria analysis. The atomic force microscopy methods will be considerably updated and adjusted for special microbiological researches.


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