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Diagnosis and Regulation of Human Intestinal Microflora

#A-732


Human Intestinal Microflora and Microbiological Aspect of Its Regulation during Diseases of Digestive Tract

Tech Area / Field

  • MED-OTH/Other/Medicine
  • BIO-MIB/Microbiology/Biotechnology

Status
8 Project completed

Registration date
24.05.2001

Completion date
01.09.2006

Senior Project Manager
Visser H

Leading Institute
Institute of Molecular Biology, Armenia, Yerevan

Collaborators

  • University of Illinois At Urbana-Champaign / Department of Animal Sciences, USA, IL, Urbana\nKarolinska Institute / Microbiology and Tumor Biology Center, Sweden, Stockholm\nJichi Medical School / Department of Surgery, Japan, Tochigi\nUniversity of Graz / University Dentistry Clinic, Austria, Graz\nMississippi University, USA, MS, 39762\nGM-Consult, Denmark, Helsinge

Project summary

The Project concerns to the development of efficient clinical bacteriological methods, suitable for large-scale use (including the improvement of methods for differentiation of several strains of intestinal microorganisms), investigation and characterisation of intestinal microflora of patients with some series of diseases of digestive tract by the use of general and new developed method, and to the elaboration of methods for the regulation of the some changes threatening to the human health.
It is known that state of host’s bacterial ecology plays an important and decisive role in formation of defined kinds of human malignant diseases. E.coli is an important representative of aerobically bacterium in human intestinal microflora. Besides the bad effects of none- pathogenic E.coli several strains on physicochemical integrity of intestine, the clinical bacteriological analyses include the determination of E.coli’s summary quantities, the quantities of E.coli strains with lack of enzymatic activities, etc, and don’t include the determination of unfavorable for human health of none- pathogenic E. coli strains in E. coli’ total quantities.
To avoid the unforeseen changes of intestinal microflora and connected with it arsenal of diseases it is also necessary improvement of clinical bacteriological methods, including the methods of indication and differentiation microorganisms’ strains.
On the other hand the analysis of literature data on the investigations of aerobically and anaerobic bacteria from human intestinal microflora has shown that till now there are no integrated approach for the regulation of intestinal microflora during the different diseases of digestive tract.
Recently treatment of disbacteriosis (microecological disbalance) and the restoration of intestine’s original ecosystem have carry out using probiotics. The method is considerably effective, however more detail studies of intestinal microflora, the studies of enzymatic activities and membranes’ structures of bacterial strains can give opportunities for the more successful use of this substances.
In this respect, the problem particularly is very actually for detection the new criterions of disbacteriosis’ description and for the regulation of the state of intestinal microflora during its unfavourable changes.
It is known that the Familial Mediterranean Fever (FMF) meets frequently, and the quantity of Armenian patients consists of large percentage in all. It is 0.25% of Armenian population and the ratio of obligate heterozygous’ quantity to health persons is 0.14. While colchicine is efficient in treating FMF, it is not without its side effects. Many patients taking colchicine complain of general gastro-intestinal upset. Therefore, the studies of different aspects of FMF, particularly the investigation of correlation between FMF and human intestinal microflora being one of the general medical problems is more important for Armenian population.
The determination of quantity and quality of several unfavourable for human health E.coli strains, total E.coli’s quantity and their ratio to quantity of definite aerobic bacteria in the gut during human bacteriological analysis can be useful not only for development of bacteriological analysis, but also for the more effective use of probiotics.
Institute of Molecular Biology of National Academy of Sciences of The Republic of Armenia (IMB) for many years together with special organisations is actively engaged in the investigations on disbacteriosis and different disease, especially the FMF and series of cancer neoplasm. In particularly the important role of E.coli G35 strains from intestinal microflora in the formation of organism’s normal state has been shown. However till now there are no data on the mechanisms of their actions, which exactly can be useful not only for regulation of bacterial activities, but also for the regulation of intestines physiological state during number of diseases.
In this context, the main objective of the Project will be the development of efficient clinical bacteriological methods, including the improvement of methods for differentiation of several strains of intestinal microorganisms, the investigation and establishment of correlationship between the state of intestinal microflora and different diseases (FMF, cancer of stomach, small and large intestine, ovaries) for the purpose to regulate the state of intestinal microflora during these diseases.
For the realization of the Project some other institutions will be involved: Department of Gastrointestinal Diseases of Republican Clinical Hospital, Department of Microbiology of Ministry of Health of Armenia and Department of Biophysics of Yerevan State University.
The project represents an integrated complex of microbiological, biochemical, biophysical, genetic and clinical studies.
Microbiological research is aimed to develop the bacterial strains identification's criterions, the quantitative and qualitative determination of Enterobacteriaceae species and its strains during bacteriological analysis; to study the peculiarities of growth and reproduction processes of bacterial strains in the aerobically and anaerobic conditions under the different states of external environment (to study the antibacterial resistance, the UV- resistance, the influence of acidity of external environment, etc.). Special studies will be devoted to the investigations of interactions between specific strains (group of strains) from human intestinal microflora.
The genetic investigations will be included specific bacterial strains characterization.
The main goals of biochemical investigation have to be the selection of criterions for laboratory diagnosis; also the studies of physico-chemical properties of bacterial membranes' structure (to study the qualitative and quantitative changes of phospholipids in different classes of biological objects during different pathological processes; to study the free radical mechanisms of damage and antioxidant protection in different classes of biological objects, etc).
The biophysical methods will be used to carry out the some physico-chemical properties of biological membranes (to study the membranes' fluidity, hydrophoby, penetration of ions, etc.).
The results expected from the Project realization are considerable scientific, practical and economical significant. These are the elaboration of new microbiological criterions for determination of the microecological disbalance’ levels, possibilities to prognosis the unfavorable for the human health changes of intestinal microflora and its regulation, the development of efficient microbiological test, suitable for large-scale use.
The following organizations may be interested in the project:
· Governmental and non-governmental departments,
· Scientific organizations that study health problems, problems of environment ecology defense, etc.


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