Antimicrobial Activities of Probiotics
Elucidation of the Mechanisms of Antimicrobial Activities of the Probiotic Strains Lactobacillus sp. and Enterococcus sp.
Tech Area / Field
- BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
3 Approved without Funding
Scientific and Research Institute of Experimental Medicine, Russia, St Petersburg
- Science and Production Firm Verta, Russia, St Petersburg
- Lawson Health Research Institute, Canada, ON, London
Project summaryThe present study is to attack the fundamental medical and biological problem that concerns lactic acid bacteria action on high organism and its microbial community.
Lactic acid bacteria (LAB), which mainly involve Lactobacilli and Enterococci spp. have been known to effectively protect high organisms from pathogens. As a component of probiotics, food supplements and food, they are profitably used for therapy and prophylaxis of dysbiosis and infections of urogenital and gastrointestinal tracts. However, the basic mechanisms of probiotic benefit action are far from being fairly understood, so the sphere of their application for prophylaxis and therapy of infections is significantly limited.
The primary goal of this proposal is to comprehensively elucidate the mechanisms of antimicrobial activities of LAB using as the examples Lactobacillus sp. and Enterococcus sp. strains that contain the genes encoding bacteriocins production. The in vitro studies of the antagonistic activities of these strains towards pathogenic and opportunistic gram-negative and gram-positive bacteria and fungi as well as the intestine and vagina healthy microflora obligate microorganisms will be performed. To elucidate the mechanisms of antimicrobial activities of these strains, the bacteriocins and the encoding genes will be isolated, analyzed and sequenced. The peptides will be synthesized by the methods of solid-phase chemical synthesis in the amounts required for further studies of bioactivity. There will be studied the antimicrobial activities of bacteriocins as well as the special features of expression in different conditions of cultivation, including those with synthetic pheromones. To evaluate the probiotics host effect, their abilities to cut the experimentally induced dysbiosis and the inflammatory process and to change the immunity parameters and functional properties of the intestine mucosa will be studied. There will be assessed: the microbial community; the congenital and acquired immunity; and glycolytic and proteolytic activities of the small and large intestine fractions (mucous surface, mucus, and chyme).
This study will be performed using up-to-date methods of molecular biology, biochemistry, genetics, physiology, bacteriology, cytology, and histology. This study will provide new knowledge on the character of the microorganism-host interaction, which offers the perspectives to the design of novel probiotics and the effective use of available microbial feed supplements and drugs.
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