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Biopreparation for Forest Protection


Technology for Obtaining Fur-Protective Biopreparation Against Spruce Bark Beetles on the Basis of Using Highly-Active Entomopathogenic Metarhizium Anisopliae Metsch Strain

Tech Area / Field

  • BIO-OTH/Other/Biotechnology
  • BIO-MIB/Microbiology/Biotechnology

3 Approved without Funding

Registration date

Leading Institute
Research Center of Toxicology and Hygienic Regulation of Biopreparations, Russia, Moscow reg., Serpukhov

Supporting institutes

  • National Academy of Sciences of the Republic of Belarus / Belarusian Institute of Plant Protection, Belarus, Minsk


  • University of Vermont / Entomology Research Laboratory, USA, VT, Burlington

Project summary

The use of ecologically safe methods for forest-protection is one of the primary elements of modern technology for phytosanitary optimization of ecosystems, including the forest ones, which make it possible to purposefully regulate the population of pests and useful species thus retaining the dynamic balance. The potential of entomopathogenic microorganisms as a means for forest protection against pest insects is rather high and their introduction into forest biocenosis makes it possible to regulate the population of phytophagans, confining the outbreaks of mass reproduction. Plant pests (spruce bark beetles, cockchafer beetle, and bark bugs) are known to be affected by entomopathogenic fungi, due to spores and conidia ability to affect insects through external covering. Spores can keep safe and survive in dead insects as well as in beddings. Dissemination of muscardene fungi in the forest biotopes is efficient against pests (including the spruce bark beetle) that winter in the forest bedding. The entomopathogenic fungi are known to have long-lasting after-effect: during the next few years following their use the infected by the preparation area is several times enlarged in comparison with the original one.

The goal of the project is development of the technology for obtaining bioinsecticide on the basis of entomopathogenic fungi Metarrhizium anisopliae Metsch strain, highly virulent for the spruce bark beetles.

Development of the technology to obtain bioinsecticide, effective for cutting down the population of spruce bark beetles will be based on the authors’ experience gained in research on entomopathogenic fungi, and the knowledge of biotechnical principles for obtaining mycoinsecticidal preparations.

We have planned to carry out search and selection of highly virulent strains of muscadene fungi M. anisopliae spp within forest biocenosis, screening on several populations spruce bark beetles for selecting the strain producer as well as development of the technology for production of biopreparation.

To achieve this objective, it is necessary to fulfill a number of subtasks. The following results are expected:

- search and isolation of pure cultures of entomopathogenic fungi Metarrhizium anisopliae Metsch strain; selection of high virulent strains on the basis of screening for spruce bark beetles;

- study of strains toxicological characteristics with respect to warm-blooded animals;

- study of physiological and biochemical characteristics, comparative commission testing of the strains, selection of optimal composition of liquid nutrient media, productivity assessment, and development of conditions for submerged cultivation;

- grounding of MPC of the strain producer in the air of working area;

- obtaining of experimental specimen of the preparation, development of screening technique; biological activity assessment on the basis of LC50 determination;

- study of toxicological-and-hygienic characteristics of the preparation;

- evaluation of biological efficiency of the preparation under field conditions for different populations of spruce bark beetles;

- ecological assessment of using the biopreparation in forest biocenosis.

Novel data on the role of entomopathogens in regulation of spruce bark beetle population in forest biocenosis will be of scientific interest from the position of balance assessment in the phytopatogen-entomopathogene system and conservation of biopersity.

Development of novel technology for obtaining ecologically safe biopreparation for protection of spruce green plantations against spruce bark beetles will be of practical interest.


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