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Bacterial and Cell Culture Collection

#2754.2


Reorganization of Bacterial and Cell Culture Collection to Study Infectious Diseases in Humans and Animals

Tech Area / Field

  • BIO-OTH/Other/Biotechnology
  • BIO-MIB/Microbiology/Biotechnology

Status
8 Project completed

Registration date
12.04.2006

Completion date
31.10.2011

Senior Project Manager
Savinova N V

Leading Institute
State Research Center for Applied Microbiology and Biotechnology, Russia, Moscow reg., Obolensk

Collaborators

  • Centers for Disease Control and Prevention (CDC), USA, GA, Atlanta\nBioterrorism Pesponse - National Microbiology Laboratory Public Health Agency of Canada, Canada, MB, Winnipeg\nPublic Health Agency of Canada, Canada, ON, Ottawa

Project summary

Correction and improvement of the Work Plant of the larger Project to increase the possibility of its financing is the major objective of this work.

In 2002 a Project “Reorganization of bacterial and cell culture Collection to study infectious diseases in humans and animals” (#2754) was submitted to ISTC. Arrangement of modern Laboratory facilities for storage of microbial and cell cultures to provide international project with standard cultures was the objective of it. In October 2004 USA and Canada decided to finance the proposal on the preparation of ISTC Project # 2754 (PDG); the total Budget made $40,000.

During the communication by E-mail, Project Collaborators made main remarks on the Project Work Plan and suggested to make detail inventory of SRCAM Culture Collection, make a review of current methods of molecular typing of microorganisms, to get knowledge of the work of Russian Collections of microorganisms, consider the requirements of physical security and biological safety of work with microorganisms.

It was suggested to discuss the Work Plan in detail during the travel of SRCAM Project Participants in USA and Canada.

Within the framework of the Project SRCAM Project Participoants visited 2 Russian Culture Collections and got knowledge about their work and structural organization, principles of making records, storage methods b collection culture testing, etc In compliance with the Work Plan, literature data on current methods of storage and typing of microorganism culture collection have been reviewed. In the result, methods of long-term storage of the collection cultures were selected, and lyophilization conditions, conditions of cryoconservation and contact sorbtion were determined. Based on the literature data analysis on molecular-genetic typing of microorganisms, the cost of appropriate equipment and reagents was estimated as well as the appropriate methods of typing were suggested.

During travel, SRCAM Project Participants got knowledge about CDC and Canadian Center for Human and Animal Diseases activity. As the results, the list of microorganisms and somatic cell lines to be studied was detailed. The list of Project participants was corrected. The list of equipment and supplies was renewed. A new Budget of the Project was provided.

The PDG activity resulted in the preparation of a new Work Plant of Larger Project #2754, which was corrected and approved by Project Collaborators. The letters of Approval were received from Project Collaborators.


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