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Hemophilus Parasuis in Swine

#2448


Diagnostics, Prevention and Typing of Hemophilus Parasuis in Swine

Tech Area / Field

  • AGR-VTH/Vaccines and Theraupetics/Agriculture
  • AGR-DIG/Diagnostics/Agriculture
  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
  • BIO-CHM/Biochemistry/Biotechnology

Status
8 Project completed

Registration date
12.03.2002

Completion date
19.02.2008

Senior Project Manager
Melnikov V G

Leading Institute
State Research Center for Applied Microbiology and Biotechnology, Russia, Moscow reg., Obolensk

Collaborators

  • Akzo Nobel / Intervet International BV, The Netherlands, Boxmeer

Project summary

Project objective: Determine different serotypes of H.parasius strains circulating in Russia, study pathogenic and virulent properties and conduct testing of anti-H.parasius vaccine provided by “Intervet” Company.
Infectious diseases in swine represent one of urgent problems of veterinary. Application of industrial technologies in animal husbandry resulted in a number of infectious diseases, which previously were beyond the attention of veterinarians. Hemofilic poliserositis in swine, H.parasuis, belongs to this group of diseases.
Spread of the diseases is observed in the regions of intensive swine breeding. Data on the incidents were reported in Yugoslavia, France, Czech Republic, Slovakiya, Switzerland, Germany, Italy, Norway, USA, Japan, Cuba, Canada, Australia, and Spain. In Russia, Prof. M.A.Sidorov, Research Institute of Experimental Veterinary, started investigations on poliserosistis in swine in 1974. During the last two years, RF Veterinary Department has reported hemofilic poliserositis incidents in Leningrad, Smolensk, Nizhnii Novgorod and Chelyabinsk regions of Russia. Within the project proposed, H.parasius was isolated in pig farms of Nizhnii Novgorod , Voronezh, Belgorod.
Since hemofilic poliserositis is observed in parallel with escherichiosis, salmonellosis, streptococcosis, etc., it can be suggested that the diseases occurs in association with bacterial and viral infections.
At present diagnostics of H.parasuis in Russia is advanced insufficiently, and in many facilities the given disease remains unnoticed on the background of other bacterial infections. Scheme of identification of H.parasuis of different serotypes has not been developed yet.
The objective of the present proposal is to develop species- and sero-specific diagnosticums to determine humoral antibodies for serological control of vaccine preparations against H.parasuis and monitoring of H.parasuis strains circulating in pig farms.
Expected results:
1. Establish a Collection of H.parasuis strains circulating in Russia.
2. Study spread of H.parasuis serotypes in different regions of RF.
3. Develop a method for isolation and serotyping of H.parasuis.
4. Study of biochemical, cultural and morphological properties of H.parasuis and its susceptibility to antibacterial preparations.
5. Develop species- and type-sepcific H.parasuis test-systems (PCR and/or ELISA)
6. Conduct field trials of H.parasuis vaccine submitted by “Intervet” Company and prepare a protocol on the efficiency of the vaccine in prevention of hemophilic poliserositis in Russia.

In order to meet the project objectives, the following tasks should be implemented:
1. Develop a Collection of H.parasuis strains.

2. Study of antigenic properties of H.parasuis isolates.

3. Prepare hyperimmune species- and type-specific rabbit sera.
4. Develop species- and type-specific test-systems
5. Conduct field trials of anti-H.parasuis vaccine submitted by “Intervet” Company

The project is directed on the study of distribution of H.parasuis infection in Russian Federation and on research for the given microorganism and creation of tests - systems facilitating screening and diagnostics of the disease, that, undoubtedly, is an urgent problem and does not contradict ISTC objectives and tasks. The project activity will involve the use of standard approaches and methods not presenting danger from the point of view of modification of the microorganisms under study, pollution of the environment and humane use of animals.
“Intervet” Company (the Netherlands) will participate in the project as a Collaborator. The Collaborator assigns the obligations of rendering the consulting assiatnce. “Intervet” Company offers a vaccine, which will be tested, within the framework of the project, in pig farms of Russia.


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