Microbial Cell - Bacteriophage Interaction
Development of Method Electrophysics Analysis for Microbial Cell - Bacteriophage Interaction
Tech Area / Field
- ENV-WPC/Water Pollution and Control/Environment
8 Project completed
Senior Project Manager
Alexandrov K A
Institute of Biochemistry and Physiology of Plants and Microorganisms, Russia, Saratov reg., Saratov
- State Research Center for Applied Microbiology and Biotechnology, Russia, Moscow reg., Obolensk
- Auburn University / Materials Research and Education Center, USA, AL, Auburn\nNew Mexico Institute of Mining and Technology, USA, NM, Albuquerque
Modern biotechnology and medicine is experienced considerable necessity in effective and low cost methods of cell detection. At the moment electrophysical methods are of special interest for determination of morphological parameters of cells and for cell detection because of the short time of analysis, the ability to implement of analytical procedures without cell destruction and the ability to create fully automated analytical procedure. Besides, this analysis is relatively cheap.
The electrooptical method deserves special attention. The method involves measuring an electrooptical effect in cell suspensions. The results of these measurements are used for determination of electrophysical and morphometrical parameters of cells and their structures. The main disadvantage of the method is the use of a limited range of cell sizes (0.5-15 mm).
There are many methods of cell detection that use bioselective agents (phages, antibodies or lectins). During past few years, methods using of selective bacteriophages as selective agents have developed greatly. The interaction of cells with bacteriophages causes changes in the dielectric properties of cells and in the electrooptic properties of cell suspensions.
The goal of project is a develop a method for cell identification with the help of an electrooptical analysis of cell suspensions, with selective bacteriophages as a selective factor.
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