Clostridium botulinum strains characterization
Genotype-phenotype characterization and toxin study of archival and newly isolated Georgian Clostridium botulinum strains
Tech Area / Field
- BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
3 Approved without Funding
National Center for Diseases Control, Georgia, Tbilisi
- Ryan Arner Science Consulting LLC, USA, TX, Freeport\nCenters for Disease Control and Prevention (CDC), USA, GA, Atlanta
Project summaryC. botulinum archival and newly isolated strains kept at Repository of NCDC will be studied by standard microbiological, molecular and protein methods using required BSL by the project personnel of the NCDC. During the last 15 years, 100 strains of C. botulinum have been isolated from clinical samples, soil and suspected food; and all strains will be considered subject to the study. To develop and apply new genetic and genomic platforms for detection and identification of unique DNA sequences in strains we will work closely with the Centers for Disease Control and Prevention, Atlanta, GA.
· US CDC will provide Real-Time PCR, MLST, and Sequencing protocols, and will assist in bioinformatics development by training Georgian scientists.
· This project will be carried out at the NCDC at the Richard G. Lugar Center with our US collaborators support.
The specific objectives of this proposal are: 1) Phenotypic characterization of archival and newly isolated strains of C. botulinum; 2) Genetic (Real-Time PCR, MLST, Sequencing) characterization of archival and newly isolated strains of C. botulinum; 3) Adapt proteomic methods to serotype Georgian strains of C. botulinum neurotoxin; 4) to identify genomic persity of C. botulinum strains in Georgia and compare with US C. botulinum strains
The International Science and Technology Center (ISTC) is an intergovernmental organization connecting scientists from Kazakhstan, Armenia, Tajikistan, Kyrgyzstan, and Georgia with their peers and research organizations in the EU, Japan, Republic of Korea, Norway and the United States.
ISTC facilitates international science projects and assists the global scientific and business community to source and engage with CIS and Georgian institutes that develop or possess an excellence of scientific know-how.