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Low Frequency Field Effects on Industrial Microbes

#A-826


The Molecular and Cellular Study of ELF EMF Effect on the Functional Activity and Biotechnological Properties of Industrial Microbes

Tech Area / Field

  • BIO-MIB/Microbiology/Biotechnology
  • BIO-CHM/Biochemistry/Biotechnology
  • BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology

Status
3 Approved without Funding

Registration date
08.11.2001

Leading Institute
UNESCO Chair in Life Sciences - Life Sciences International Educational Center, Armenia, Yerevan

Supporting institutes

  • Yerevan State University, Armenia, Yerevan\nArmenian State Agrarian University, Armenia, Yerevan\nInstitute of Radiophysics and Electronics, Armenia, Ashtarak-2

Collaborators

  • Polyclinique Maymard/Service de Cancerologie Medicale et de Radiotherapie, France, Bastia

Project summary

The ELF EMF effect on the functional activity of microbes is known for a rather long time. It was shown that EMF has frequency dependent modulation effect on the growth of yeasts (Dardanoni et al., 1985; Grundler & Kaiser, 1992) and blue-green algae (Tambiev & Kirikova, 1992). At present the ELF EMF are applied in microbiotechnology and namely in food biotechnology (Beglarian et al., 1990; Beglarian, 1986, 1996). The ELF EMF induced modulation effect can be revealed on cell morphology, biopolymers syntheses, membrane function, cell proliferation activity, enzyme activity, the level of antibiotics production and so on (Pachomov et a.l, 1998). Our previous studies of the ELF EMF effect on the functional activity of E.coli and Lactobacteria 4-10 Hz frequency windows have been revealed when the functional activity is modulated by ELF EMF more effectively. At the same time the similar frequency dependent effects on the E.coli K-12 functional activity were shown in the case when simply the solutions were treated by ELF EMF. A hypothesis has been suggested stating that the ionic transporting mechanisms in membrane realized by carriers are the extrasensitive targets for the ELF EMF effect. It is suggested that the proton pump can serve as such a mechanism in microbe’s membrane. The purpose of the present project is:



(1) to study the ELF EMF frequency dependent effect on cell function depending on the proton pump activity by investigating the microbe membrane sensitivity to 0-20Hz EMF at different intracellular levels of cyclic nucleotides,
(2) to work out the optimal conditions of the ELF EMF induced activation of microorganisms with the aim of future application of these properties in food production biotechnology by

a) studying the ELF EMF frequency dependant effect on the functional activity of industrial microorganisms and on the products quality characteristics,
b) studying the possible changes of bakery yeast and Lactobacteria technological characteristics,
elaboration of recommendations for improving the microbiological technologies by using the selected frequency EMF in bakery and diary production.


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