Genetic Polymorphism of HIV-1
Genetic Polymorphism of HIV-1 in the Former USSR Republics
Tech Area / Field
- BIO-CGM/Cytology, Genetics and Molecular Biology/Biotechnology
8 Project completed
Senior Project Manager
Melnikov V G
Ivanovsky Institute of Virology, Russia, Moscow
- Republic Center for Prevention and Avoidance of AIDS Infection, Kazakstan, Almaty\nRepublica Association "AIDS", Kyrgyzstan, Bishkek\nArmenian National AIDS Prevention Center, Armenia, Yerevan
- University of Amsterdam / Academic Medical Center, The Netherlands, Amsterdam
Project summaryThe HIV-infection epidemic has been rapidly spreading in Russia as well as other former Soviet Union republics. Most of these countries start the national HAART programs. The effectiveness of these programs will depend substantially on choosing the adequate means of disease control and surveillance. Of considerable importance in the right choice of such means are genetic properties of HIV-1 circulating in the regions.
Our study will focus on genetic persity of HIV-1 variants circulating in Armenia, Kazakhstan, and Kyrgyzstan. The main technical approaches to be applied include collecting samples of serum and blood cells from HIV-infected persons, serological assays, heteroduplex mobility assay, RNA and DNA sequencing, phylogenetic analysis. The molecular epidemiological studies will provide an insight into the prevalence of HIV-1 subtypes as well as frequency of transmission of HIV-1 strains bearing mutations associated with drug resistance. At present the conventional molecular monitoring of HIV-infection epidemic in Russia as well as the study of mutations associated with HIV-1 drug resistance is performed in the laboratory of T-lymphotropic viruses of Ivanovsky Institute of virology, Moscow. This program is supported and funded by the Russian Ministry of Health as a part of the Federal special program for HIV-infection prophylaxis.